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Microalgae are small, simple eukaryotic organisms with the ability to grow and produce lipids using sunlight. Due to their significant lipids content and their high efficiency in biomass production, they represent an ideal and renewable source of biodiesel-grade lipids. Microalgae biotechnology has several potential advantages over terrestrial crops, such as simple cell structure and cultivation and ease of cultivation, which can be facilitated by the use of bioreactors. However, some major challenges need to be overcome before they can be employed for mass production of microalgal biodiesel. The first and most important is their lipid content, which is usually between 35 and 60% (w/w). The second challenge is the extraction of high-quality biodiesel with low levels of impurities. The third challenge is the relatively low amount of microalgal biomass. Based on the results obtained in the past years, it appears that the best strategy to improve lipid accumulation is the genetic modification of microalgae to enhance the production of lipid and/or the discovery of microalgal strains with increased lipid content. In this review, the current status of microalgae 0b46394aab
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